Whereas all of Broly's blasts have a green coloration, the Bio-Hyper Beam is distinctly red in color.
Bio-Broly uses this technique twice in Dragon Ball Z: Bio-Broly; once against Android 18 and later against Lord Jaguar and Men-Men. After Goten left Trunks alone against Bio-Broly to save people from the Culture Fluid, Android 18 punches the Bio-Warrior in the face in an attempt to help Trunks and Bio-Broly responds by firing his Bio-Hyper Beam at her. Later, after Goten once again attempted to leave the battle and was hit by Bio-Broly's Mouth Energy Wave, Lord Jaguar asks Bio-Broly, who is now crushing Trunks, to stop his rampage and Bio-Broly responds with his Bio-Hyper Beam, destroying the stairs Jaguar and his cousin were on.
Video Game Appearances
In Super Dragon Ball Heroes: World Mission, it is named Bio Paralyze Beam in the English localization. Additionally there is a Bio Paralyze Beam Accessory Sticker that can be used to add the Super Attack to a custom character card or equipped to an Hero, Elite, or Berserker type character card in a custom deck (Special Types cannot equip accessories). As a result it can be used as a Super Attack by more characters than Bio-Broly, though this applies to all Super Attack accessories.